alpha-SMA Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: ACTA2 GeneID: 59 Uniprot: P62736 Specificity: alpha-SMA Antibody detects endogenous levels of total alpha-SMA. Immunogen: A synthesized peptide derived from human alpha-SMA, corresponding to a region within N-terminal amino acids. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 15 months from date of receipt. Reactivity: Human,Mouse,Rat,Bovine Prediction: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken Application: WB,IHC,IF/ICC,IP,ELISA(peptide) Dilution: WB: 1: 500-1: 1000, IHC: 1: 50-1: 500, IF/ICC: 1: 200, IP 1: 100, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2835329 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF1032-BP Des# Blocking Peptide: alpha-SMA Blocking peptide