Phospho-Histone H2A (Thr121) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: HIST1H2AB; HIST1H2AE,HIST1H2AH,HIST1H2AD,HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM,HIST2H2AA3; HIST2H2AA4,HIST3H2A GeneID: 3012,8335,85235,3013,8329,8330,8332,8336,8969,723790,8337,92815 Uniprot: P0C0S8/P04908/P20671/Q96KK5/Q6FI13/Q7L7L0 Specificity: Phospho-Histone H2A (Thr121) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Threonine 121. Immunogen: A synthesized peptide derived from human Histone H2A around the phosphorylation site of Thr121. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat Prediction: Bovine,Xenopus Application: WB,IHC,ELISA(peptide) Dilution: IHC 1: 50-1: 200, WB 1: 1000, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2834570 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF3135-BP Des# Blocking Peptide: Phospho-Histone H2A (Thr121) Blocking peptide