Phospho-ERK1/2 (Thr177/Thr160) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: MAPK3,MAPK1 GeneID: 5595,5594 Uniprot: P27361/P28482 Specificity: Phospho-ERK1/2 (Thr177/Thr160) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr177/Thr160. Immunogen: A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Thr177/Thr160. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat Prediction: Application: IHC,IF/ICC,ELISA(peptide) Dilution: IHC 1: 50-1: 200, IF/ICC 1: 100-1: 500, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2847001 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF3687-BP Des# Blocking Peptide: Phospho-ERK1/2 (Thr177/Thr160) Blocking peptide