Phospho-EPHB1/2/3/4 (Tyr600/Tyr602/Tyr614/Tyr596) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: EPHB4,EPHB3,EPHB2,EPHB1 GeneID: 2050,2049,2048,2047 Uniprot: P54762/P29323/P54753/P54760 Specificity: Phospho-EPHB1/2/3/4 (Tyr600/Tyr602/Tyr614/Tyr596) Antibody detects endogenous levels of EPHB1/2/3/4 only when phosphorylated at Tyr600/602/614/596. Immunogen: A synthesized peptide derived from human EPHB1/2/3/4 around the phosphorylation site of Tyr600/602/614/596. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat Prediction: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken Application: WB,ELISA(peptide) Dilution: WB 1: 1000-3000, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2840207 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF8145-BP Des# Blocking Peptide: Phospho-EPHB1/2/3/4 (Tyr600/Tyr602/Tyr614/Tyr596) Blocking peptide