Phospho-Histone H2A (Ser2) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: HIST1H2AA,HIST1H2AB; HIST1H2AE,HIST1H2AC,HIST2H2AB,HIST1H2AD,HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM,HIST2H2AC,HIST2H2AA3; HIST2H2AA4,HIST1H2AJ,HIST3H2A GeneID: 221613,3012,8335,8334,317772,3013,8329,8330,8332,8336,8969,8338,723790,8337,8331,92815 Uniprot: P04908/P0C0S8/P20671/Q16777/Q6FI13/Q7L7L0/Q8IUE6/Q93077/Q96QV6/Q99878 Specificity: Phospho-Histone H2A (Ser2) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Ser2. Immunogen: A synthesized peptide derived from human Histone H2A around the phosphorylation site of Ser2. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat Prediction: Application: WB,ELISA(peptide) Dilution: WB 1: 1000-3000, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2840285 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF8223-BP Des# Blocking Peptide: Phospho-Histone H2A (Ser2) Blocking peptide