Phospho-Bim (Thr56/Thr116) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: BCL2L11 GeneID: 10018 Uniprot: O43521 Specificity: Phospho-Bim (Thr56/Thr116) Antibody detects endogenous levels of Bim only when phosphorylated at Thr116(Isoform BimEL, O43521-1) or Thr56(Isoform BimL, O43521-2). Immunogen: A synthesized peptide derived from human Bim around the phosphorylation site of Thr56/116. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat Prediction: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus Application: WB,ELISA(peptide) Dilution: WB 1: 1000-3000, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2840309 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF8247-BP Des# Blocking Peptide: Phospho-Bim (Thr56/Thr116) Blocking peptide