Phospho-Flt3 / CD135 (Tyr955) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: FLT3 GeneID: 2322 Uniprot: P36888 Specificity: Phospho-Flt3 / CD135 (Tyr955) Antibody detects endogenous levels of Flt3 / CD135 only when phosphorylated at Tyr955. Immunogen: A synthesized peptide derived from human Flt3 / CD135 around the phosphorylation site of Tyr955. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Monkey Prediction: Pig,Bovine,Horse,Sheep,Rabbit,Dog Application: WB,IF/ICC,ELISA(peptide) Dilution: WB 1: 1000-3000, IF/ICC 1: 100-1: 500, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2840531 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF8477-BP Des# Blocking Peptide: Phospho-Flt3 / CD135 (Tyr955) Blocking peptide