Recombinant Human Ribonuclease H1(RNASEH1)

Volume: 20ug. Other sizes are also available. For further information, please contact us. Research Areas: Epigenetics and Nuclear Signaling Uniprot ID: O60930 Gene Names: RNASEH1 Organism: Homo sapiens (Human) AA Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED Expression Region: 1-286aa Sequence Info: Full Length Source: E.coli Tag Info: N-terminal 6xHis-tagged MW: 37.6 kDa Alternative Name(s): Ribonuclease H type II RNH1 Relevance: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed: 10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II Reference: "Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination." Skourti-Stathaki K., Proudfoot N.J., Gromak N. Mol. Cell 42: 794-805(2011) Purity: Greater than 85% as determined by SDS-PAGE. Form: Liquid or Lyophilized powder Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Function: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids Involvement in disease: Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2) Subcellular Location: Cytoplasm Protein Families: RNase H family Tissue Specificity: Ubiquitous. Paythway: HGNC Database Link: https: //www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC: 18466 UniGene Database Link: https: //www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=568006 KEGG Database Link: https: //www.genome.jp/dbget-bin/www_bget?hsa: 246243 STRING Database Link: https: //string-db.org/network/9606.ENSP00000313350 OMIM Database Link: https: //www.omim.org/entry/604123604123604123 Lead Time Guidance: 3-7 business days