Phospho-WEE1 (Ser642) Antibody

Size : 100 µL Clone Number: Aliases: DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; Wee1 homolog antibody; WEE1 homolog S. pombe antibody; Wee1 like protein kinase antibody; Wee1 tyrosine kinase antibody; Wee1+ homolog antibody; Wee1+ S. pombe homolog antibody; WEE1, S. pombe, homolog of antibody; WEE1, somatic antibody; Wee1-like protein kinase antibody; WEE1_HUMAN antibody; WEE1A antibody; Wee1A kinase antibody; WEE1hu antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P30291 Immunogen: Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, WB; WB: 1: 500-1: 1000 Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Watanabe N., EMBO J. 14: 1878-1891(1995). Cichutek A., Cytogenet. Cell Genet. 93: 277-283(2001). Igarashi M., Nature 353: 80-83(1991) Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: WEE1 Research Areas: Epigenetics and Nuclear Signaling;Cell biology