Phospho-BLM (Thr99) Antibody

Size : 100 µL Clone Number: Aliases: Blm antibody; BLM_HUMAN antibody; Bloom syndrome antibody; Bloom syndrome protein antibody; Bloom syndrome RecQ helicase like antibody; BS antibody; DNA helicase antibody; DNA helicase RecQ like type 2 antibody; MGC126616 antibody; MGC131618 antibody; MGC131620 antibody; RECQ 2 antibody; RECQ like antibody; RecQ like type 2 antibody; RecQ protein like 3 antibody; RecQ protein-like 3 antibody; RecQ-like type 2 antibody; RecQ2 antibody; RECQL 2 antibody; RECQL 3 antibody; RECQL2 antibody; RECQL3 antibody; type 2 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P54132 Immunogen: Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome. Host: Rabbit Reactivity: Human Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 1000, IHC: 1: 50-1: 100 Background: Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). Ellis N.A., Cell 83: 655-666(1995). Karow J.K., J. Biol. Chem. 272: 30611-30614(1997). Pichierri P., EMBO J. 23: 3154-3163(2004). Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: BLM Research Areas: Epigenetics and Nuclear Signaling