Phospho-EIF2S1 (Ser49) Antibody

Size : 100 µL Clone Number: Aliases: EIF 2 alpha antibody; EIF 2 antibody; EIF 2A antibody; EIF 2alpha antibody; eIF-2-alpha antibody; eIF-2A antibody; EIF-2alpha antibody; EIF2 alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha antibody; Eukaryotic translation initiation factor 2 subunit 1 antibody; Eukaryotic translation initiation factor 2 subunit alpha antibody; IF2A_HUMAN antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P05198 Immunogen: Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, WB, IHC, IF; WB: 1: 500-1: 1000, IHC: 1: 50-1: 100, IF: 1: 100-1: 200 Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: EIF2S1 Research Areas: Epigenetics and Nuclear Signaling