Phospho-CAD (Thr456) Antibody

Size : 100 µL Clone Number: Aliases: CAD antibody; CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase antibody; EC 6.3.5.5); Aspartate carbamoyltransferase antibody; EC 2.1.3.2); Dihydroorotase antibody; EC 3.5.2.3)] antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P27708 Immunogen: Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD. Host: Rabbit Reactivity: Human, Mouse Tested Applications: ELISA, IHC; IHC: 1: 50-1: 100 Background: The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Iwahana H., Biochem. Biophys. Res. Commun. 219: 249-255(1996). Davidson J.N., DNA Cell Biol. 9: 667-676(1990). Olsen J.V., Cell 127: 635-648(2006). Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: CAD Research Areas: Epigenetics and Nuclear Signaling;Cancer;Cell biology;Metabolism