Phospho-CASP9 (Thr125) Antibody

Size : 100 µL Clone Number: Aliases: APAF-3 antibody; Apoptotic protease Mch-6 antibody; Apoptotic protease-activating factor 3 antibody; CASP-9 antibody; CASP9 antibody; CASP9_HUMAN antibody; Caspase 9 antibody; Caspase-9 subunit p10 antibody; ICE-LAP6 antibody; ICE-like apoptotic protease 6 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P55211 Immunogen: Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 1000, IHC: 1: 50-1: 100 Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. Duan H., J. Biol. Chem. 271: 16720-16724(1996). Srinivasula S.M., J. Biol. Chem. 271: 27099-27106(1996). Hadano S., Mamm. Genome 10: 757-760(1999) Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: CASP9 Research Areas: Cancer;Cell biology;Metabolism