Phospho-IKBKB (Tyr199) Antibody

Size : 100 µL Clone Number: Aliases: I kappa B kinase 2 antibody; I kappa B kinase beta antibody; I-kappa-B kinase 2 antibody; I-kappa-B-kinase beta antibody; IkBKB antibody; IKK beta antibody; IKK-B antibody; IKK-beta antibody; IKK2 antibody; IKKB antibody; IKKB_HUMAN antibody; IMD15 antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta antibody; NFKBIKB antibody; Nuclear factor NF-kappa-B inhibitor kinase beta antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: O14920 Immunogen: Peptide sequence around phosphorylation site of tyrosine 199(Q-K-Y(p)-T-V)derived from Human IKK-?. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, IF; IF: 1: 100-1: 200 Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: IKBKB Research Areas: Cancer Cardiovascular?Cell biology;Immunology;Signal transduction