Phospho-EIF4G1 (Ser1232) Antibody

Size : 100 µL Clone Number: Aliases: DKFZp686A1451 antibody; eIF 4 gamma 1 antibody; eIF 4G 1 antibody; eIF 4G1 antibody; eIF-4-gamma 1 antibody; eIF-4G 1 antibody; eIF-4G1 antibody; EIF4 gamma antibody; EIF4F antibody; EIF4G antibody; EIF4G1 antibody; EIF4GI antibody; Eukaryotic translation initiation factor 4 gamma 1 antibody; IF4G1_HUMAN antibody; p220 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: Q04637 Immunogen: Peptide sequence around phosphorylation site of serine 1232 (P-V-S(p)-P-L) derived from Human eIF4G. Host: Rabbit Reactivity: Human Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 1000, IHC: 1: 50-1: 100 Background: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis De Gregorio, E. et al. (1998) RNA 4, 828-836. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136. Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: EIF4G1 Research Areas: Epigenetics and Nuclear Signaling