Phospho-Histone H3.3 (T3) Antibody

Size : 50 µL Clone Number: 28H4 Aliases: Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B Product Type: Recombinant Antibody Immunogen Species: Human UniProt ID: P84243 Immunogen: A synthesized peptide Host: Reactivity: Human, Mouse Tested Applications: ELISA, WB, ICC, FC; Recommended dilution: WB: 1: 500-1: 5000, ICC: 1: 50-1: 500 Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Clonality: Monoclonal Isotype: Rabbit IgG Purification Method: Affinity-chromatography Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: Liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: H3F3A Research Areas: Epigenetics and Nuclear Signaling