Phospho-HSF1 (S326) Antibody

Size : 50 µL Clone Number: 2A7 Aliases: Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSF1 Product Type: Recombinant Antibody Immunogen Species: Human UniProt ID: Q00613 Immunogen: A synthesized peptide derived from human Phospho-HSF1 (S326) Host: Reactivity: Human Tested Applications: ELISA, WB, IHC; Recommended dilution: WB: 1: 500-1: 5000, IHC: 1: 50-1: 200 Background: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed: 1871105, PubMed: 11447121, PubMed: 1986252, PubMed: 7760831, PubMed: 7623826, PubMed: 8946918, PubMed: 8940068, PubMed: 9341107, PubMed: 9121459, PubMed: 9727490, PubMed: 9499401, PubMed: 9535852, PubMed: 12659875, PubMed: 12917326, PubMed: 15016915, PubMed: 25963659, PubMed: 26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed: 9727490, PubMed: 11583998, PubMed: 16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed: 1871105, PubMed: 1986252, PubMed: 8455624, PubMed: 7935471, PubMed: 7623826, PubMed: 8940068, PubMed: 9727490, PubMed: 9499401, PubMed: 10359787, PubMed: 11583998, PubMed: 12659875, PubMed: 16278218, PubMed: 25963659, PubMed: 26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed: 11583998, PubMed: 16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed: 1986252, PubMed: 26727489). Binds to chromatin at heat shock gene promoters (PubMed: 25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed: 9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed: 14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed: 17897941). Plays a role in the regulation of mitotic progression (PubMed: 18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed: 26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed: 26754925). Clonality: Monoclonal Isotype: Rabbit IgG Purification Method: Affinity-chromatography Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: Liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: HSF1 Research Areas: Tags & Cell Markers