Plasmid DNA Extraction Maxi Kit (10prep), FavorFilter, EndotoxinFree, Ion Exchange

25% OFF list price as part of our FALL Sale! On now until October 18th, 2024! DESCRIPTION The FavorFilter Endotoxin Free Plasmid Extraction Maxi Kit (Maxiprep) is designed for the quick isolation of endotoxin-free purified plasmid DNA using anion-exchange technology. This kit uses the FavorFilter Maxi Cartridge to clarify the lysate; the gravity-flow column has improved DNA binding capacity. PTR Buffer washes away the endotoxins in just one step. This kit is designed for the convenient, easy, and efficient extraction of pure plasmid DNA and makes the endotoxins less than 0.05 EU/µg DNA that is suitable for the transfection of cultured cells. FEATURES Time Saving: Remove bacterial lysates without centrifugation. High Purity: Endotoxin <0.05 EU/µg DNA, ideal for endotoxin, Equal to 2× CsCl gradient centrifugation method. Safety: Eliminate the use of phenol, chloroform, ethidium bromide, and cesium chloride; minimizing expose to hazardous materials. SPECIFICATIONS Format/Principle: Anion-exchange chromatography (Gravity-flow column) Lysate Clarification: Filtration Input Sample Volume: 120~240 ml of bacterial culture for high/low-copy high-copy number or low-copy number plasmids. Procedure time: <120 minutes Plasmid or Constructs Range: 3 kbp~150 kbp Binding Capacity: 1.5 mg/Maxi Prep Column APPLICATIONS Transfection (for endotoxin sensitive cells); microinjection; in vitro transcription; in vitro transcription; restriction enzyme digestion. PROCEDURE In the process, the cell pellet is resuspended (PM1; RNase A contained), lysed (PM2), and neutralized (PM3). Next, the filter cartridge is used to remove bacterial lysates and obtain cleared sample mixture. Then, the plasmid DNA/buffer mixture bind to the ion exchange resin inside the PM Maxi Column; the impurities are removed by PW Buffer. Finally, the purified plasmid DNA is eluted using high-salt PEL Buffer and precipitated with isopropanol for desalting. KIT COMPOSITION PEQ Buffer PM1 Buffer PM2 Buffer PM3 Buffer PTR Buffer PW Buffer PEL Buffer RNase A (Lyophilized) FavorFilter Maxi Cartridges PM Maxi Columns