HEK293 cells stably expressing Alpha-1A adrenergic Receptor, Calcium biosensor & ß-arrestin biosensor

Description Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and two Gentaur Biosensors to monitor G-protein and β-arrestin modulation. Each vial of Gentaur α1A-adrenoceptor Cell Line contains HEK293 cells stably expressing the following constructs: human α1A-adrenoceptor with no tag Calcium Gentaur Biosensor (Green Fluorescence) β-arrestin Gentaur Biosensor (Red Fluorescence) Gentaur α1A-adrenoceptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to ADRA1A a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.