U2OS Cell Line stably expressing GLP-1 Glucagon Receptor & cAMP Biosensor

Description cAMP Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Gentaur Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP Gentaur GLP-1 Receptor (GLP1R) Cell Line contains U2OS cells stably expressing the following constructs: human GLP-1 Receptor (GLP1R) with no tag; cAMP Gentaur Biosensor (Red Fluorescence). cAMP Gentaur GLP-1 Receptor (GLP1R) Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to GLP-1, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.