EP1 Prostanoid Receptor Cell Line

Description Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and two Gentaur Biosensors to monitor G-protein and β-arrestin modulation. Each vial of Gentaur EP1 Prostanoid Receptor Cell Line contains U2OS cells stably expressing the following constructs: human EP1 Prostanoid Receptor with no tag Calcium Gentaur Biosensor (Green Fluorescence) b-arrestin Gentaur Biosensor (Red Fluorescence) Gentaur EP1 Prostanoid Receptor Cell Line allows to assay compounds analyzing both signaling pathways involving receptor activation; both G protein by Ca2+ flux and b-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to EP1 Prostanoid Receptor both signalling pathways are activated which, in turn, triggers a cellular response mediated by calcium and b-arrestin recruitment. This leads to an increase of fluorescence intensity of both b-arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.